![]() Yuryev, A.) (Humana Press, Totowa, NJ, 2007). The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: twenty-something years on. Preparation of genomic DNA from mammalian tissue. Single-step PCR optimization using touchdown and stepdown PCR programming. Type-specific amplification of viral DNA using touchdown and hot start PCR. Molecular identification and immunolocalization of the water channel protein aquaporin 1 in CBCECs. Combining restriction digestion and touchdown PCR permits detection of trace isoforms of histamine H3 receptor. Analysis of circadian liver gene expression by ADDER, a highly sensitive method for the display of differentially expressed mRNAs. Kornmann, B., Preitner, N., Rifat, D., Fleury-Olela, F.Mapping transposon insertion sites by touchdown PCR and hybrid degenerate primers. Levano-Garcia, J., Verjovski-Almeida, S.Use of degenerate primers and touchdown PCR to amplify a halogenase gene fragment from Streptomyces venezuelae ISP5230. ![]() Use of degenerate primers and touchdown PCR for construction of cDNA libraries. Comparison of single and touchdown PCR protocols for detecting Pneumocystis jirovecii DNA in paraffin-embedded lung tissue samples. The use of 'touchdown' polymerase chain reaction increases the sensitivity and specificity of t(11 14)(q13 q32) detection in patients with mantle cell lymphoma. Multistep-touchdown vectorette-PCR-a rapid technique for the identification of IVS in genes. Single-tube heminested PCR coupled with 'touchdown' PCR for the analysis of the walleye dermal sarcoma virus env gene. High and low annealing temperatures increase both specificity and yield in touchdown and stepdown PCR. Improved thermodynamic parameters and helix initiation factor to predict stability of DNA duplexes.
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